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生理状态下外周血白细胞IL-2基因表达的荧光定量PCR检测 被引量：3

Quantitative Real-time Detection of Spontaneous Interleukin 2 Gene Expression in Peripheral Blood Mononuclear Cell by Polymerase Chain Reaction
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摘要 目的:建立白细胞介素2(IL-2)基因表达的绝对定量实时PCR检测技术。方法:应用实时定量PCR方法检测25名健康运动员中IL-2mRNA的表达。主要结果和结论:应用实时定量PCR方法能够对健康受试者外周血白细胞自发性IL-2mRNA表达进行定量分析,相关系数r>0.99。 Objective To develop a method for quantitative real-time detection of interleukin 2(IL-2) using polymerase chain reaction (PCR). Methods Gene expression of IL-2 in peripheral blood mononuclear cell (PBMC) from 25 healthy individuals was detected by PCR. An endogenous “housekeeping' gene GAPDH was used to normalize the results. Results and Conclusion The spontaneous IL-2 mRNA expression in PBMC in a given sample could be measured by PCR with relativity correlation coefficient of r>0.99.
作者 王茹 陈佩杰 董强刚
机构地区 上海体育学院运动科学系
出处 《中国运动医学杂志》 CAS CSCD 北大核心 2005年第4期457-459,475,共4页 Chinese Journal of Sports Medicine
基金 国家科技攻关计划(2002BA904B04-6) 上海市教委第四期重点学科--运动人体科学学科建设项目 国家教育部高等学校优秀青年教师教学科研奖励计划 上海市体育局科研攻关项目资助
关键词 外周血白细胞 荧光定量PCR检测 基因表达 生理状态 实时定量PCR方法 IL-2MRNA PCR检测技术 mRNA表达 白细胞介素 健康受试者 绝对定量 定量分析 相关系数 运动员 自发性 interleukin 2, mRNA, real-time PCR
分类号 R446.9 [医药卫生—诊断学]
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参考文献14

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二级参考文献16

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共引文献61

同被引文献39

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二级引证文献39

中国运动医学杂志
2005年 第4期

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