摘要
目的 建立家兔血浆中酮康唑(ketoconazole)浓度的高效液相色谱检测法。方法 采用HypersilODSC18柱,以甲醇 水 三乙胺(79:2 0 :1)为流动相,紫外检测波长2 4 1nm ,安定为内标,乙醚提取,浓缩后进样。结果 酮康唑与内标分离良好;血浆酮康唑浓度在0 .5～10 0 .0 μg/ml范围内线性良好,r =0 .9996 ;最低检测浓度为0 .2μg/ml;0 .5、5 .0、5 0 .0 μg/ml三种浓度的平均回收率分别为91.2 %、96 .9%和98.7% (n =5 ) ;日内和日间RSD <10 %。结论 该方法操作简便、可靠、取血量少,适用于酮康唑的血药浓度测定和临床生物利用度的研究。
Objective To establish a HPLC method for the determination of ketoconazole level in plasma ofrabbit. Methods An agilent 1100 instrument with Hypersil C18 column（5μm,4.6 mm×250mm） was used.The mobile phase was a mixture of methonal, water and triethylamine in a ratio of 79：20 ： 1 （adjusting pH to 6.6 by hydrochloric acid） at a flow rate of 1.0 ml/min. The ultraviolet detector was set at 241nm. Results A good linear range of ketoconazole was obtained from 0.5 to 100μg/ml（ r = 0. 9996）, and the detection limit was 0.2μg/ml. The average recovery rate（ n = 5 ）at the concen-tration of 0.5, 5.0 and 50ug/ml was 91.2%,96.9% and 98.7%, respectively. Conclusion The method was simple, accurate and reliable. It is suitable for the determination of ketoconazole level in plasma and can also be used for bioavailability study.
出处
《皖南医学院学报》
CAS
2005年第3期227-229,共3页
Journal of Wannan Medical College
