摘要
目的:探讨广西籍壮族IgA肾病(IgAN)与HLA-DR基因的相关性,并进行序列分析。方法:采用聚合酶链反应—序列特异性引物法(PCR-SSP)对25例广西籍壮族IgAN患者及50名正常对照的HLA-DR4、HLA-DR12基因频率进行检测,并测定等位基因序列。结果:壮族IgAN组HLA-DR4的基因频率高于对照组(P<0.05),HLA-DR4基因阳性组持续性镜下血尿伴蛋白尿的发生率高于阴性组(P<0.05)。1例IgAN患者的HLA-DRB1*04基因出现碱基对变异,149位A→G,临床表现为发作性肉眼血尿,病理类型为局灶节段性肾小球硬化。结论:1HLA-DR4基因可能是广西籍壮族IgAN的易感基因;2HLA-DR4基因与临床表现为持续性镜下血尿伴蛋白尿的广西籍壮族IgAN相关。
Objective：To evaluate relationship between HLA and IgA nephropathy,we study HLA-DR gene susceptibility to IgA nephropathy in Guangxi Zhuang population. Methods ：HLA-DR4 and HLA-DR12 genes in 25 cases of IgA nephropathy patients and 50 normal controls were investigated by Polymerse Chain Reaction-Sequence Specific Primers（PCR-SSP） technique. Sequences of HLA-DR4/HLA-DR12 genes were analysed by direct sequencing of PCR products. Result： The frequency of HLA-DR4 gene in IgA nephropathy patients was significantly higher than that in normal controls（ P ＜0.05）;and proteinuria is more frequent in HLA-DR4-position patients than that in HLA-DR4-negative patients （P ＜ 0.05）. Conclusion：HLA-DR4 gene may be susceptible allele in patients with IgA nephropathy in Guangxi Zhuang population,and HLA-DR4 gene is associated with clinical presentation.
出处
《广西医科大学学报》
CAS
北大核心
2005年第3期332-335,共4页
Journal of Guangxi Medical University
基金
广西科学基金资助项目(No.桂科基0156022)
关键词
IGA肾病
HLA—DR基因
PCR—SSP
序列分析
IgA nephropathy
HLA-DR gene
Polymerse Chain Reaction-Sequence Specific Primers（PCR-SSP）
sequence analysis
