摘要
本试验以10个大麦基因型3种大小不同的幼胚为材料,研究了它们在含有3、4、5毫克2,4-D的培养基上的培养反应.结果发现:1.不同基因型的出愈率,愈伤组织平均鲜重以及直接出苗和直接出芽的频率都有一定的差异.2.所有基因型的出愈率都是随着幼胚的增大而提高,除个别基因型例外,大多数基因型的愈伤组织平均鲜重、直接出苗和直接出芽的频率也随幼胚的增大而提高.3.2,4-D浓度对出愈和直接出芽的影响较少,但2,4-D浓度增加有抑制愈伤组织增重,促进直接出苗的作用,4.盾片与培养基接触能促进出愈和抑制直接出苗,本试验共获得三大类愈伤组织,综上所述,大胚、低2,4-D浓度、盾片与培养基接触是大麦幼胚培养中获得愈伤组织高产的关键.
Three kinds of immature embryos of ten barley genotypes were cultured on B5 media containing 3 mg/1, 4 mg/1 and 5 mg/1 2,4 -D respectively in order to study the influence of embryo size, 2,4-D concentration and genotype on calli induction. The results showed: (1)The induction rate, fresh weight of calli, and the rate of direct emergence and germination were different amony genotypes, and rose with incresse of emoryo size extept a few genotypes (2) The effect of 2,4-D concentration on canlli induction and direct mergence of seeding was very little, but the increse of 2,4-D concentrati on could inhibit the multiplication of aclli and enhance the direct emergence of seedlings. (3) Three kinds of calli were obtained in this study. To sum up, big embryo, 1QW concentration of 2,4-D and scutellum being in contact with the culture media were very important for obtaining high yield of calli in culture of barley immature embryos.
关键词
大麦
离体培养
愈伤组织诱导
幼胚
barley
in vitro culture
callus induction
immature embryo
