摘要
以基因枪轰击后的6个大麦品种的幼胚为材料,研究了不同质量浓度的ABA,2,4-D,ZT和IAA对胚幼的出愈率、愈伤组织分化特性和植株再生能力的影响。结果表明:用1 0mg/L的ABA在诱导培养基上处理幼胚能大大降低胚芽鞘发生率,并对出愈率和胚性愈伤组织的形成无不良影响;经1 0mg/L的2,4-D诱导产生的胚性愈伤组织转到分化培养基上以后,芽分化率可达8%～17%,而4 0mg/L2,4-D处理芽分化率极低。1 0mg/LZT与0 1mg/LIAA配比能降低分化过程中不定根的发生率,有利于芽的分化。通过植株再生系统的优化,6个大麦品种的转化幼胚植株再生率达到2%～8%。
Effects of different ABA,2,4-D concentrations and ZT and IAA ratio on callus induction and plant regeneration of barley immature embryos,bombed by microprojectile,were studied.The results demonstrated that 1.0 mg/L ABA could effectively inhibit coleoptile growth of immature embryo without negative effects.Frequency of bud differentiation was up to 8%～17% from embryonic callus induced by the treatment of 1.0 mg/L 2,4-D,while few buds induced by 4.0 mg/L 2,4-D.Desirable combination of ZT and IAA for bud differentiation was 1.0 mg/L ZT and 0.1 mg/L IAA.According to optimum hormone concentrations,plant regeneration frequency of bombed immature embryos from six barley varieties were at a range of 2%-8%.
出处
《华北农学报》
CSCD
北大核心
2003年第4期1-4,共4页
Acta Agriculturae Boreali-Sinica
基金
国家948项目(991028)
关键词
基因枪
大麦幼胚
组织培养
植株再生
质量浓度
Microprojectile
Barley immature embryos
Hormone
Callus
Plant regeneration.
