摘要
实现了葡萄糖氧化酶以及葡萄糖氧化酶和电子传递媒体Fe(CN)_6^(3-)同时在纳米级金膜微带电极上的固定.用红外光谱和循环伏安对GOD/PPy微电极进行了表征,研究了微带金电极上聚吡咯恒电位形成过程的动力学及葡萄糖氧化酶对其动力学过程的影响.探讨了微酶电极GOD/Fe(CN)_6^(3-)/PPy对葡萄糖氧化的催化作用.考察了PPy膜厚度和溶液中氧的存在对GOD/Fe(CN)_6^(3-)/PPy微电极测定葡萄糖的影响。
The immobilization of glucose oxidase, as well as simultaneous immobilization of glucose oxidase and Fe(CN)63- as an electron mediator in polypyrrole film was successfully accomplished at a nanometer-sized ultramicroband gold electrode to provide a simple biosensor for detecting glucose without mediator in solution. The GOD/ PPy electrode was characterized by IR spectrum and cyclic voltammetry. The electropolymerization process of pyrrole and the effects of glucose oxidase on the electropolymerization pyrrole were also studied with chronoamperometry. The effects of polypyrrole thickness and oxygen in solution on the determination of glucose at GOD / Fe(CN)63- / PPy microelectrode were discussed.
出处
《化学学报》
SCIE
CAS
CSCD
北大核心
1995年第3期281-285,共5页
Acta Chimica Sinica
基金
国家自然科学基金
青年基金资助课题
