摘要
培养在液体培养基中的尖顶肚菌(Morchella conica Pers)菌丝体可以分离出大量的原生质体。在组合的2号酶液中,在30℃下经4.5小时处理的产量最高(为6.2×10~6个原生质体/100mg·ml)。此法分离得的原生质体再生力很强,液体培养18小时即可再生成菌丝。再生的细胞进行植板培养后形成了菌丝体,并获得了从原生质体再生的菌株。
Protoplasts of Morchclla conica Pers were isolated from the mycelia cultured in liquid medium. The highest isolated quantity was 6.2×10^(6) protoplasts 100mg·ml in mixed enzyme solution No. 2 and at 30℃for 4.5 h in cubation. Isolated protoplasts regenerated to hyphae readily. After 18 hours of incubation hyphae grew out from the cells regenerated from the protoplasts. Following plating on solid medium regenerated isolates were obtained.
出处
《云南植物研究》
CSCD
1989年第4期449-452,共4页
Acta Botanica Yunnanica
关键词
尖顶羊肚菌
原生质体
菌丝体再生
Morchella conica
Protoplasts
Mycelium regeneration
