摘要
用胶原酶分离豚鼠肾上腺髓质嗜铬细胞，获得离体豚鼠肾上腺髓质嗜铬细胞悬液，可用作刺激－分泌偶联研究的模型．２．２ｍｍｏｌ／ＬＣａ２＋促进嗜铬细胞儿茶酚胺的分泌，与无钙组比较有显著差异，不同浓度的川芎嗪（１０－２～１０－７ｍｏｌ／Ｌ）与Ｃａ２＋（２．２ｍｍｏｌ／Ｌ）的混合液作用于细胞悬液，细胞儿茶酚胺的分泌受混合液中Ｃａ２＋的促进，而川芎嗪无显著影响．在细胞悬液中，加入川芎嗪（１０－３～１０－９ｍｏｌ／Ｌ）５ｍｉｎ后，再加入Ｃａ２＋（２．２ｍｍｏｌ／Ｌ）作用５ｍｉｎ，Ｃａ２＋对嗜铬细胞儿茶酚胺分泌的促进作用受到抑制，其中川芎嗪浓度为１０－６ｍｏｌ／Ｌ时，抑制作用最为明显．
Guinea pig adrenal gland chromaffin cells were isolated by collagenase(mg/mL) digestion.After a short time (5 ￣ 8 hours) cultivation in buffer Ⅱcontaining 5 mg/mL bovine serum ablumin, chromaffin cell suspensions wereobtained and could be used as the model of stimulus-secretion eoupling.2. 2 mmol/L Ca2+ stimulates the releasing of catecholamine of chromaffin cells.Comparing with free Ca2+, the difference is significance: t= 26. 76>t30,0.01= 2. 75.Cell suspensions were mixed with Ligustrazine (10-2 mol/L￣10-7 mol/L) and Ca2+(2. 2 mmol/L), release of catecholamine evoked by Ca2+, were not affected byLigustrazine. After cell suspensions were mixed with Ligustrazine (10-3 mol/L10-9 mol/L) 5 minutes, then mixed with Ca2+ (2. 2 mmol/L) 5 minutes, thecatecholamine release evoked by Ca2+ is inhibited by Ligustrazine. WhenLigustrazine concentration is 10-6 mol/L, the inhibitory action is more obvious.
出处
《华中师范大学学报（自然科学版）》
CAS
CSCD
1995年第4期489-493,共5页
Journal of Central China Normal University：Natural Sciences
关键词
嗜铬细胞
川芎嗪
钙
儿茶酚胺
分泌
抑制效应
Guinea pig
adrenomedullary chromaffin cell
Ca￣(2+)
Ligustrazine
stimulus-secretion coupling
