摘要
采用聚丙烯酰胺凝胶电泳对石刁柏（ＡｓｐａｒａｇｕｓｏｆｆｉｃｉｎａｌｉｓＬ．）雌雄植株不同器官及不同组织的过氧化物酶同工酶谱进行了研究，结果表明，除根部外，尽管鳞片、茎尖、拟叶等器官酶谱有明显差异，但雌雄株间差异有相同的趋势。雄株均比相应的雌株少一条酶带，雌雄植株组织培养获得的愈伤组织和茎尖过氧化物酶同工酶酶谱差异也有类似的规律。说明石刁柏性别差异与过氧化物酶同工酶的数目有关，过氧化物酶同工酶谱的差异可以作为性别鉴定的指标。
Isozymes of peroxidase from different sexual asparagus (Asparagus offictinalis L. )plants were analysed by polyacrylamide gel electrophoresis. The result showed that, with the exception of roots, the enzyme diversity on scaly leaf, stem apex and cladode appeared in both male and female plants, and the enzyme bands in male plants were less than that in female plants. The isozymes of peroxidase on callus and stern apex from tissue culture showed same result as above. The results indicated that sex of asparagus plants has relationship with their enzyme patterns. Therefore, the latter can be used to identify sex of asparagus plants.
出处
《华北农学报》
CSCD
北大核心
1995年第2期67-71,共5页
Acta Agriculturae Boreali-Sinica
关键词
石刁柏
雌株
雄株
同工酶
Asparagus
Male plant
Female plant
Isozymes of peroxidase
