摘要
本文通过对条斑紫菜Ｒ－ＰＥ（藻红蛋白）及其α－β－γ亚基的吸收光谱和荧光光谱进行计算机解叠，研究了Ｒ－ＰＥ内发色团之间的能量传递过程，并对Ｒ－ＰＥ及亚基内的各发色团进行了“ｓ”和“ｆ”型的指认。发现在亚基中为“ｆ”型的发色团在Ｒ－ｐＥ（αβ）＿６γ中起着“ｓ”型发色团的作用，且将能量传递给最后的“ｆ”型发色团。荧光激发偏振光谱进一步证明了Ｒ－ＰＥ内的能量转移过程与计算机解叠的结果一致。
The energy transfer processes ih R-phycoerythrin as well as its α,β,γ,subunits isolatedfrom red alga prophyra yezoensis were studied by absorption, fluorescence spectra and thedeconvolution of these spectra.A tentative assignment of“s”and“f”type chromophores in R-PE and α,β,γ subunits was also maken, The“f”type chromophore in β(or γ)subunitis found to be an s-type chromophore in R-PE[(αβ)_6γ], and transfer its energy to the final“f”type chromophores. The results obtained from the fluorescence excitation polarizationspectrum of R-PE are in good agreement with the deconvolution results for the energy transfer processes.
出处
《感光科学与光化学》
CSCD
1995年第1期53-60,共8页
Photographic Science and Photochemistry
基金
"八五"重大基础研究项目
国家自然科学基金
关键词
光谱解叠
能量传递
藻胆蛋白
红藻
条斑紫菜
deconvolution, energy transfer,phycobiliprotein To whom correspondence should be addressed.
