摘要
本文用多克隆抗角蛋白抗体,以免疫过氧化酶PAP法,对128例不同类型肿瘤的常规石蜡包埋组织进行标记。其结果大多数癌除肝细胞肝癌、肾透明细胞癌外,大部分肿瘤细胞显示中等度到强阳性。在滑膜肉瘤、恶性间皮瘤及胸腺瘤上皮样成份弱阳性。黑色素瘤、恶性淋巴廇、恶性纤维组织细胞瘤、上皮样平滑肌瘤、精原细胞瘤和类癌都显阴性。结果提示该多克隆抗角蛋白抗体在低分化或未分化癌与淋巴瘤、黑色素瘤等鉴别诊断中是有价值的。
Specimens from 128 human tomors of various types are marked in routinely paraffin-embedded tissues by PAP three stage immunoperoxidase technics with a polyclonal antikeratin antibody(PAKA).The majority of the carcinomas,except hepatocellular,adrenocortical clear cell ones are stained of strong or moderate intensity in the majority of tumor cells.The similar results are obtained in synovial sarcoma,malignant mesothelioma.and thymoma.Malignant melanoma,malignant lymphoma,malignant fibrous histocytoma.and myoleioblastoma,seminoma,carcinoid tumor do not show any reactivity 'with PAKA.The results suggest that PAKA is valuable in diagnostic distinction of poorly or undifferentiated carcinoma from lymphoma,melanoma.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1989年第4期270-273,共4页
Journal of Third Military Medical University
关键词
肿瘤
角蛋白
标记
免疫酶技术
诊断
neoplasms/ID
keratin/DU
immunoenzyme technics
immunochemistry
antibodies
keratin marking
polyclonal antibody
