摘要
目的：探讨哮喘患者外周血嗜酸细胞（ＥＣ）的氧化代谢活性及活性氧产生与正常人的差别，并了解重要致喘介质血小板激活因子（ＰＡＦ）对它的影响。方法：采用鲁米诺放大的化学发光法。２２例哮喘患者及２５例正常人各分为两组，均从外周血中分离出ＥＣ，Ⅰ组的ＥＣ用酵母多糖刺激，Ⅱ组的ＥＣ用ＰＡＦ刺激，最后用生物化学发光仪检测发光值。结果：哮喘患者血ＥＣ受ＰＡＦ刺激后化学发光较正常人增强，５×１０￣５细胞的发光峰值分别为（１．１５±０．３６）×１０￣５ｍｉｎ￣（－１）和（０．５６±１０．３１）×１０￣５ｍｉｎ”（Ｐ＜０．０５）。结论：哮喘患者外周血ＥＣ处于激活状态，对ＰＡＦ的反应增强。
Objective:To study the difference of the blood eosinophils oxidative
metabolic activity and perox-ide production between the asthmatics and the normal;and the
influence of the inflammation mediatorplatelet activating factor(PAF )on them. Methods:The
technique of luminol- enhanced chemiluminescencewas used.Twenty-two asthma patients and
twenty- five normal were devided into two groups,respectively.One group was stimulated with
opsonized zymosan,the other with PAF,and the number of counts perminute was
registered.Result:We found that eosinophils from the asthmatics produced a
significantlygreater chemiluminescence induced by PAF[(1.15 ±0. 36) ×10￣5 min￣(-1)/5 ×
10￣5 cell]than normal cells did[(0.56±0. 31)×105 min￣(-1)/5 ×10￣5 cell],P<0.
05.Conclusion:The results suggested that the eosinophilsfrom the asthmatics are in activative
state and hyperresponsive to PAF.
出处
《第四军医大学学报》
1995年第6期437-439,共3页
Journal of the Fourth Military Medical University
关键词
哮喘
嗜酸细胞
血小板激活因子
化学发光
astbma
eosinophil
platelet activating
factor
chemiluminescence
