摘要
利用我国中药温莪术提取的抗癌有效部位榄香烯（ｅｌｅｍｅｎｅ）合并加温，对Ｈ２２－Ｆ２５／Ｌ小鼠肝癌细胞进行体外抑癌实验。结果显示：Ｅｌｅｍｅｎｅ（５０ｕｇ／ｍｌ）或加温（≥３９０℃）均对肝癌细胞有抑癌作用，并且随着加温温度的上升或时间的延长抑癌作用愈益明显。其中以Ｅｌｅｍｅｎｅ（５０ｕｇ／ｍｌ）合并加温（４３０℃）持续１小时抑癌效果显著。其３Ｈ－ＴｄＲ癌细胞掺入率仅为０．２４％，核分裂明显抑制（Ｐ＜０．０１），作用后的癌细胞不能再传代。这结果说明Ｅｌｅｍｅｎｅ合并加温对Ｈ２２－Ｆ２５／Ｌ小鼠肝癌细胞在体外有显著的抑癌作用，说明该药具有临床热化疗前景。
Elemene,extracted from the chinese medical herb curcuma wenyujin and hyperthermia was used for antitumor action on H22-F25/L hepatomas.The results showed that in vitro elemene(50μg/ml) or hyperthermia(≥39℃) had inhibitory cancer effects.But the strongest effect expressed 3H-TDR uptake rate only 0.42%,obvious division inhibitory rate(P<0.01) and losing reproductive capacity when elemene(50μg/ml) combined with heat(43℃) simultaneously for 60 minutes.The above mentioned experimental results suggested that elemene combined with heat(acted synergistieally) had the most significances of antitumor action on H22-F25/L hepatomas.In addition,elemene has no severe side effects that other anticancer drugs have.The report provides the basis for further clinical application of intraperitoneal hyperthermia with elemene in abdominal cancers.
出处
《大连医科大学学报》
CAS
1995年第1期16-19,共4页
Journal of Dalian Medical University
