摘要
在电镜下观察了苏云金芽胞杆菌菌株ＣＴ－４３的芽胞萌发、芽胞和伴胞晶体的形成过程。芽胞萌发分活化、萌动和脱出３个阶段。芽胞形成分为轴丝形成、前芽胞隔膜形成、前芽胞形成、初生细胞壁形成、芽胞外套形成、芽胞衣片层形成和芽胞形成７个阶段。伴胞晶体形成从芽胞形成第２阶段开始，由一些小结晶聚集而成。晶体镶嵌在菌株ＣＴ－４３中很普遍。结合实验对伴胞晶体形成的调控和外膜的形成等进行了讨论。
Continual thin section from Bacillus thuringiensis strain CT-43 germination and sporulation showed that germination of CT-43 spore was divided into 3 stages,that were activation, germination, and outgrowth. The sporulation was divided into 7 stages which included the formation of axial filament(8h) , forespore septum(8～9h) ,incipient spore(9～10 h) , cortex & primordial cell wall(10～11h), exosporium(11～12 h), lamellar spore coat(13～14h), and outer coat(14h). The formation of parasporal crystal was initiated by forespore septum. Also, a few cells could form parasporal crystal before septum and some mature cells contained crystal only. Parasporal crystal was assembling by small crystalline and mosaic crystal was frequenlly observed. The regulation of parasporal crystal formation and the two-layer membrane formation of parasporal crystal are discussed.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
1995年第1期64-68,T003,共6页
Journal of Huazhong Agricultural University
关键词
苏云金芽胞杆菌
菌株
芽胞萌发
芽胞形成
Bacillus thuringiensis
strain CT-43
germination
sporulation
parasporal crystal
