摘要
本文应用透射电镜技术对２０例新鲜手术切除胃癌及癌旁粘膜、４例正常胃粘膜的神经内分泌（ＮＥ）细胞进行观察，并与抗ＣｇＡ抗体及９种激素抗体免疫组化技术进行对比观察。电镜下正常胃泌酸区粘膜可见ＥＣＬ细胞、Ｐ细胞、ＥＣ细胞、Ｘ细胞，偶见Ｄ细胞；胃窦粘膜可见Ｇ细胞、Ｄ细胞、ＥＣ细胞。２０例胃癌中含ＮＥ型癌细胞８例，依据内分泌颗粒的形态不同可分为三种类型。１６例癌旁粘膜ＮＥ细胞的形态与正常相似，但数量有明显变化。对正常胃粘膜ＮＥ细胞的超微形态与功能，胃癌中ＮＥ细胞超微诊断特点及临床病理意义进行了探讨。
The neuroendocrine (NE) cells in fresh specimens of gastric carcinomas and gastric mucosa adjacent to the carcinomas taken from 20 cases and normal gastric mucosa taken from 4 subjects were observed with transmission electron microscopy (TEM) and their immunohistochemical characteristics were contrasted by using antiCgA and nine kinds of hormonal antibodies. The ECL cells, P cells, EC cells , X cells and a few D cells in oxyntic mucosa and G cells,D cells, EC cells in antrum mucosa could be seen under TEM. Carcinomatous NE cells were found in 8 out of 20 cases with gastric carcinoma. Such NE cells with other types of carcinoma cells formed neoplastic glandular structures and nests infiltrating into the stroma. It is shown that the NE cellular and nuclear shapes were heteromorphic and based on their different shapes of endocrine granules, the NE cells could be divided into three types. In the cytoplasm of some NE carcinomatous cells contained both NE and mucous granules. The shape of NE cells in gastric mucosae adjacent to the carcinomas was similar to that of the normal, but the number varied remarkably. The diagnostic features of ultrastructural pathological changes of NE cells and their functional alterations in gastric carcinoma were discussed.
出处
《诊断病理学杂志》
CSCD
1995年第2期76-78,共3页
Chinese Journal of Diagnostic Pathology
关键词
胃癌
内分泌细胞
透射电镜
免疫组化
诊断
Gastric carcinoma
Gastric mucosa
Neuroendocrine cells
Transmission electron microscopy
Immunohistochemistry
