摘要
血清转铁蛋白微小变异的出现，可能是酒精性肝病重要的生化学指标之一。但由于检测方法复杂，临床应用受到限制。应用ｐＨ３．５～９．５和ｐＨ４．０～６．０的安福林溶液等量混合，并除去血清白蛋白后行等电聚焦电泳，染色后得到清晰变异带。并用此法检测了酒精性肝病和非酒精性肝病患者血清，结果前者的阳性率为６６．７％～８３．３％，后者仅为１６．７％。由于该方法，操作简单，用时较短便于临床应用。
Appearance of serum desialo-transferrin(De-TF)has been emphasized as
an important marker for alcoholic liver diseaso(ALD);however. the
clinical application was limited because of the complexation of
detecting methed.In these study,serum De-TF bands can be easily
detectable by simple protein staining:when isoelectric focussing of
Serum was performed using a mixed SolutiOn of equal amounts of two
Ampholines(pH3.5～9.5 and 4.0～6.0)after removing albumin. The
positive rate was higher in patients with ALD(66.7～88.3%)than in
those with non-ALD(decompensated liver cirrosls,16.7%).In this study,
we have developcd a simple and fast method for detection of
microheterogeneity of Serum transferrin in clinics.
出处
《肝脏病杂志》
CSCD
1995年第4期214-215,共2页
关键词
转铁蛋白
微小变异
酒精性肝病
诊断
TranSeferin Microheterogeneity Alcohollic liver disease
