摘要
应用放射配体结合分析，检测尿毒症患者外周血多形核白细胞（ＰＭＬ）的糖皮质激素受体（ＧＲ），尿毒症患者ＰＭＬＧＲ的最大结合容量（Ｂｍａｘ）明显低于对照组，而其解离常数（Ｋｄ）比对照组的显著升高。同时检测ＧＲ的效应指标：皮质醇（Ｆ）对ＰＭＬ的趋化移动（ＣｈｔＭ）的抑制率（ＦＩ），Ｆ对ＣｈｔＭ的ＦＩ明显低于对照组，与降低的ＧＲ呈正相关（ｒ＝０．７８５，Ｐ＜０．０１）．尿毒症患者ＰＭＬ的ＧＲ降低的同时，伴效应指标的改变，表明ＧＲ的降低具有临床意义。尿毒症患者ＰＭＬ的随机移动（ＲＭ）和ＣｈｔＭ均明显降低，提示白细胞功能降低。
Glucocorticoid receptor (GR) of human peripheral polymorphonuclear leukocytes (PML)from thirty patients with uremia were investigated with[3H]dexamethasone as radioligand to determine the maximal binding capacity (Bmax) and dissociation constant (Kd) of GR, which were 3508±598 site/cell and 11. 7±2.6nmol/L, respectively and were significantly different from those of the controls (7250±1006site/cell, P<0.01 and 5.3±1.9nmol/L, P<0.05, respectively). Random migration (RM) and chemotactic migration (ChtM) of PML in uremic patients were significantly decreased as compared with those of the healthy subjects. The fraction inhibition (FI) of ChtM by hydrocortisone was lower in patients than in the normal persons and was positively correlated with the lowered Bmax of GR on PML(r=0. 785,P<0.01), indicating that the reduced GR binding sites on PML of uremic patients were accompanied by the lowered migratory function of PML,i. e. the effector parameter and may have implication in the development of the disease.
出处
《标记免疫分析与临床》
CAS
1995年第3期125-128,共4页
Labeled Immunoassays and Clinical Medicine
关键词
尿毒症
受体
糖皮质激素
趋化性
多形核白细胞
Uremia
Neutrophils
Receptors
glucocorticoid
Hydrocortisone
Chemotaxis
