摘要
本文以中药提取物羧甲基茯苓多糖为IFN－α的促诱生刘，以研究其对IFN促诱生效应。本研究以献血员白细胞为诱生细胞，以自制IFN－α为启动诱生剂，以新城鸡瘟病毒为诱生剂，以不同浓度羧甲基茯苓多糖为促资生剂。结果表明，常规诱生组IFN－α效价为0．735×104IU／ml，启动诱生组效价为1．127×104IU／ml，羧甲基茯苓多糖促诱生组效价为2.24×104IU／ml，是常规诱生组的3倍。不同浓度的羧甲基茯苓多糖促诱生IFN以50μg／ml为佳。羧甲基茯苓多糖促诱生IFN在37℃条件下，4～20h逐渐上升，20～24h达高峰，然后逐渐下降。
The accelerating inductive effect to interferon (IFN) by Carboxymethyl Pachymaran (CMP) was reported. The inductive cells were human WBC,the initiation inductive agents were IFN-α and the inductive agents were Nawcast disease viruses (NDV). The results indicate that the titers of IFN-α in the common group,initiation group and CMP group were 0. 735×104, 1.127 × 104 and 2. 24 × 104IU/ml,respectively. The titer of CMP group was three time higher than that of common group. 50μg/ml CMP was good for accelerate inductive effect.
出处
《合肥医学院学报》
1995年第1期8-10,共3页
Journal of Zunyi Medical University
关键词
羧甲基茯苓多糖
干扰素
诱生效应
interferon inductive effect Carboxymethyl Pachmaran
