摘要
【目的】探讨连翘酯苷作用于感染PCV2的小鼠模型后,对IFN-α和Mx1表达的影响,以评价连翘酯苷的抗病毒作用。【方法】复制PCV2感染小鼠模型。以(2、4、10mg·mL-1)连翘酯苷分为高、中、低3个剂量组,并设空白对照组和病毒对照组。给药12、24、36h后,分别提取小鼠肺组织总RNA和总蛋白,应用实时荧光定量PCR检测IFN-α、Mx1 mRNA含量,采用Western blotting检测Mx1蛋白的表达水平。【结果】空白对照组小鼠肺组织中Mx1和IFN-α无表达,病毒对照组少量表达。随着连翘酯苷浓度的升高,IFN-α和Mx1 mRNA水平逐步升高,两者的表达量呈现平行相关;高剂量组中IFN-α和Mx1表达量最高(P<0.001);随着药物作用时间的延长表达量降低,12h时达到最高(P<0.001),其后逐渐下降。【结论】昆明小鼠适于PCV2感染模型的建立。中药连翘酯苷能显著上调IFN-α和Mx1的表达,并在给予高剂量药物后12h发挥显著的抗病毒作用。Mx1蛋白与病毒的感染密切相关,可用于病毒感染的早期诊断,及具有较好的应用前景。
【Objective】 The purpose of the experiment is to research the effects of forsythoside A on the expression of IFN-α and Mx1 in the mouse model of PCV2 infection, and further to evaluate the antiviral activity of forsythoside A. 【Method】 The mouse models were established by infection with PCV2 and then divided into low, medium and high-dose groups, which were treated with 2, 4, 10 mg·mL^-1 forsythoside A respectively, the blank control group and virus control group. Total RNA and total protein were extracted from the lung tissue of mouse in each group after 12, 24, 36 h administration. The mRNA content of IFN-α and Mx1 were detected by Real-time fluorescence quantitative PCR, and the expression of Mx1 protein was detected by Western blotting. 【Result】 IFN-α and Mx1 were no expressed in the lung tissue of mouse in the blank control group, and less expression was observed in the virus control group. With the concentration of forsythoside A increasing, the mRNA content of IFN-α and Mx1 gradually increased and parallelly correlated with each other. The highest expression of IFN-α and Mx1 was observed in the high-dose group （P〈0.001）, the expression of IFN-α and Mx1 gradually decreased following the extension of time and reached the maximum after 12 h high-dose drug administration （P〈0.001）. 【Conclusion】 The data showed that Kunming mice is suitable for the establishment of model which was infected with PCV2. Forsythoside A could promote the expression of IFN-α and Mx1 significantly, and played a significant antiviral activity after 12 h high-dose drug administration. It is concluded that Mx1 protein is closely related to the virus infection, and it can be used for early diagnosis of viral infection.
出处
《中国农业科学》
CAS
CSCD
北大核心
2010年第15期3237-3243,共7页
Scientia Agricultura Sinica
基金
国家自然科学基金(30771625
30972215)
北京市自然科学基金(6092004)
北京市属高校人才强教计划(PHR2009-07135)
