摘要
以组织氧耗量与琥珀酸脱氢酶为指标,并采用火箭免疫电泳及单相免疫扩散抗原性免疫学检测方法,旨在探讨人、豚鼠新鲜皮肤与不同冷冻温度下皮肤活力及抗原性的变化。结果表明:用这两种指标百分比与平均活力百分比进行比较中,以-196℃速冻组平均活力%最高(70.8%,61%),与其它三个温度组比较有显著或非常显著意义(P<0.05,或0.01)。检测皮片抗原性的结果显示:新鲜皮片匀浆抗原性(量)>-196℃处理组>-80℃处理组>-20℃处理组>4℃处理组。由此可见,经各种低温处理的皮片匀浆抗原性(量)均有不同程度的降低,且温度越高,抗原性越低,即抗原性低的组织失活就越重。
Oxygen consumption and succinate dehydrogenase contents were deter- mined to compare mean viabilities(%)of human cadaver and guinea pig skin stored un- der different temperatures.4C,—20 C ,—80 C,and—196C.The results showed that the mean viability(%) of skin cryopreserved under—196C was superior to those under 4 C,—20 C,and —80 C (70.8%,61% respectively for —196 C ,P<0.05 or 0.01).Mi- croimmunoelectrophoresis and rocket immunoelectrophoresis were performed for related antigens and antibodies.The results of microimmunoelectrophoresis showed that the antigenicities were different for four groups.Antigenicity of fresh skin was highest.The higher the storage temperature,the lower the antigenicity of skin was.
关键词
冷冻保存
皮片组织
活力
抗原性
Freeze
Skin preservation
Skin viability
Skin antigenicity
