摘要
应用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE 电泳)分析了兔皮中Ⅲ型胶原的含量。采用此法检测了正常兔皮伤后72h 深Ⅱ度创面、伤后1月及1.5月的已愈创面组织中胶原成分的改变,旨在探讨深Ⅱ度烧伤早期及愈后皮肤胶原的变化,结果表明创面在伤后及愈合后组织中Ⅲ型胶原含量均显著减少。提示在创面愈合过程中,胶原修复的同时伴有Ⅲ型胶原的减少。
Type Ⅲ collagen was determined by SDS-polyacrylamide gel elec- trophoresis in normal rabbit skin,skin with deep Ⅱ° burn 72h after injury,skin one month after burn,and healed burn 1.5 month after injury.Results showed that there was a reduction of type Ⅲ collagen in the process of healing of burn wounds.
