摘要
应用原位杂交的方法，结合血小板释放产物β－ＴＧ和ＰＦ＿４测定及巨核祖细胞体外培养，对３３例慢性ＩＴＰ患者骨髓巨核细胞中原癌基因ｃ－ｍｙｃ和ｃ－ｓｉｓ的表达及其与β－ＴＧ、ＰＦ＿４和ＣＦＵ－ＭＫ的关系进行了研究。发现，巨核细胞ｃ－ｓｉｓ表达较对照组明显降低，ｃ－ｍｙｃ表达无显著差异。ｃ－ｓｉｓ表达与β－ＴＧ和ＰＦ＿４含量呈负相关。ｃ－ｍｙｃ表达与ＣＦＵ－ＭＫ呈正相关。ＭＫ数增高组的ＣＦＵ－ＭＫ正常，ｃ－ｍｙｃ表达增高，而ＭＫ数正常组的ｃ－ｍｙｃ、ｃ－ｓｉｓ表达和ＣＦＵ－ＭＫ均降低，提示多数ＩＴＰ患者骨髓巨核细胞增生，伴有成熟障碍，少数患者巨核细胞的增殖能力降低。
he mRNA expression of c-myc and c-sis onco-genes in bone marrow megakaryorytes (MK) and itscorrelation with β-TG, PF_4 and CFU-MK were assayedin 33 ITP patients by the method of in situ hybridiza-tion. The expression of csis mRNA was depressed inbone marrow MK in ITP patients as cornpared with thatin controls, while there was no difference in the expres-sion of c-myc mRNA between the two groups. The in-crease of &TG and PF. levels showed a negative correla-tion with the expression of c-sis, while the decrease ofCFU-MK number showed a positive correlation with theexpression of cmyc in bone marrow MK. In the groupwith increased number of MK but normal number ofCFU-MK, the expression of c-myc in bone marrow MKwas elevated , while in the group with normal number ofMK, the expression of cmyc and c-sis as well as thenumber of CFU-MK was declined. It suggested that inmost of ITP patients the bone marrow MK were hyper-plasia with a defective maturation , but in a few cases,there was an impairment in the proliferative capacity ofMK.
出处
《中华血液学杂志》
CSCD
北大核心
1995年第3期122-124,共3页
Chinese Journal of Hematology
基金
胡应洲基金
关键词
紫癜
血小板减少
巨核细胞
基因表达
原位杂交
ITP Megakarayocyte Gene ex-pression
oncogene In situ hybridiza-tion
