摘要
应用系列稀释法ＰＣＲ技术检测含５％携带ＴＣＲｙ基因重排的ＣＣＲＦ－ＣＥＭ株细胞的模拟缓解期白血病骨髓的微量残留白血病细胞，敏感性为１０￣＿（－５）～１０￣（－６）水平。经ＶＰＬ加ＡＤＭ、Ｖｐ１６、ＶＣＲ联合体外净化１小时，结果显示有３个对数级的杀伤作用。用体外克隆形成培养法观察该联合方案对急性期白血病患者白血病祖细胞集落（ＣＦＵ－Ｌ）及正常骨髓造血干细胞集落（ＣＦＵ－ＧＭ）的抑制作用，ＣＦＵ－ＧＭ存活率为２８．０２％，而ＣＦＵ－Ｌ为３．９５％（Ｐ＜０．０１），提示有显著的选择性净化作用。
he residual leukemic cells before and after ex vivopurging were detected by serial dilution-polymerasechain reaction (PCR ) , and a 3-log killing effect was ob-served when bone marrow contained 5% CCRF-CEMcells was exposed to verapamil plus vincristine,adromycin and etopside for one hour. By in vitro cultureof colony formation ,it was observed that 28. 02% ofGM-CFU was spared while 96.05% of AML-CFU elim-inated, suggesting a remarkable purging effect.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
1995年第1期23-25,共3页
Chinese Journal of Hematology
关键词
骨髓移植
自体
白血病
聚合酶链反应
e Bone marrow transplantation, autologous Minimal residual leukemic cellsPurging Polymerase chain reaction
