摘要
作者通过体外骨髓半固体和长期液体培养（ＬＴＢＭＣ），研究了干扰素－ａ（ＩＦＮ－ａ）对慢性粒细胞白血病（ＣＭＬ）细胞的作用。在半固体琼脂培养体系中，ＩＦＮ－ａ能抑制正常对照和ＣＭＬ患者７天和１０天ＣＦＵ－ＧＭ，而不抑制１４天及其后的ＣＦＵ－ＧＭ。在ＬＴＢＭＣ中，于培养开始只加一次ＩＦＮ－ａ（１０￣２Ｕ／ｍｌ，１０￣３Ｕ／ｍｌ，１０￣４Ｕ／ｍｌ），对非贴壁层细胞数和基质细胞层的形成均无明显影响，但大剂量ＩＦＮ－ａ（１０￣３Ｕ／ｍｌ和１０￣４Ｕ／ｍｌ组）对非贴壁层的ＣＦＵ－ＧＭ抑制明显；每周连续加用ＩＦＮ－ａ（１０Ｕ／ｍｌ，１０￣２Ｕ／ｍｌ，１０￣３Ｕ／ｍｌ），对非贴壁层细胞数和ＣＦＵ－ＧＭ均有抑制作用，而且ＩＦＮ－ａ浓度愈高，贴壁层的形成愈少。上述结果表明：ＩＦＮ－ａ优先抑制晚期ＣＦＵ－ＧＭ，对基质细胞也有一定抑制作用。
he in vitro effect of interferon-a(IFNa) on chronicmyelogenous leukemia (CML) were assayed in semi-solid and longterm bone marrow culture (LTBMC).In semi-solid agar culture systems IFN-a showed in-hibitory effects on day 7 and day 10 CFU-GM growthof normal and CML bone marrow , but little or no ef-fects on day 14 or later CFU-GM growth. In LTBMC ,there showed no inhibition on the cell number of non-adherent layer and the formation of adherent layer ifIFN-a was added only once at the initiation of the cul-ture, but the large dosage of IFN-a (10￣3U/ml and10￣4U/ml) showed significant inhibitory effects onCFU-GM growth of non-adherent layer. The inhibi-tion on the cell number of nona-dherent layer and theformation of adherent layer was observed when IFN-a(10U/ml,10￣2U/ml , 10￣3U/ml) was added weekly , andthe higher concentration of IFN-a was added , the few-er of the adherent layer was formed. It suggested thatIFN-a selectively inhibited the late CFU-GM and thedevelopment of stromal cells .
出处
《中华血液学杂志》
CAS
CSCD
北大核心
1995年第7期353-355,共3页
Chinese Journal of Hematology
基金
中国医学科学院协和医科大学基金
