摘要
采用组织培养和单层细胞培养两种方法对ＳＤ大鼠的胰腺进行培养。在培养过程中，培养液上清中免疫活性胰岛素（ＩＲＩ）含量均处于较高水平，培养１５天后不仅其胞内胰岛素含量分别为＞８００ｎｇ／４０ｍｇ组织和＞８００ｎｇ／１０７个细胞，而且所得的胰岛细胞对葡萄糖刺激具有灵敏的应答能力。组织培养１３天、单层细胞培养７天后，培养液中即难以检出淀粉酶含量，可见这两种方法均能获得生长良好的胰岛细胞，且其胰岛素生物合成能力和应答能力均处于旺盛状态。
The tissue culture and the single layer cell culture techniques for SD rat pancreatic islets were studied.During 15 days of incubation,the insulin concentrations in supernate were high and the concentrations in islets were>800ng/40mg tissue and>800ng/107 cells respectively.The response to glucose of the islet cells abtained after incubation was sensitive.Amylase in supernate were difficult to be detected after 11 days of tissue culture or 5days of single layer cell culture.The results indicated that we could obtaine the islets with uigorous response and insulin synthesis by both tissue and single layer cell culture techiques.
出处
《中华器官移植杂志》
CAS
CSCD
1995年第4期158-160,共3页
Chinese Journal of Organ Transplantation
