摘要
目的:建立通脉降脂胶囊(姜黄,大黄,女贞子,泽泻)的质量标准。方法:采用薄层层析法对供试品中姜黄素,大黄酸,大黄素,大黄酚和芦荟大黄素进行了鉴别;采用高效液相色谱法对供试品的姜黄素,游离大黄素,游离大黄酚进行了含量测定。结果:用C18柱,流动相为甲醇水醋酸(77∶22∶1),检测波长为428nm,测定结果线性关系良好;姜黄素、大黄素、大黄酚的平均回收率分别为101.8;97.55;97.64;每粒胶囊中各成分平均含量为姜黄素2.27mg,游离大黄素为0.162mg,游离大黄酚为0.291mg。结论:含量测定方法简便,准确,重复性好,适用于通脉降脂胶囊的质量控制。
AIM The quality standard of Tongmai Jiangzhi Capsules (Rhizoma Curcumae Longae, Radix et Rhizoma Rhei, Fructus Ligustri Lucidi, Rhizoma Alismatis) has been established. METHODS TLC was used to identify curcumin, rhein, emodin, chrysophanol and aloe-emodin. HPLC was used to determine the content of curcumin, free-chrysophanol and free-emodin. RESULTS[WTBZ]: The linear relation was excellent for determination with ODS column and methanol-water-acetic acid(77∶ 22∶ 1)as mobile phase. The average recoveries of curcumin, free-emodin, free-chrysophanol were 101.8 % , 97.55 % and 97.64 % , respectively. The curcumin content in capsule was 2.27 mg, free-emodin 0.162 mg, free-chrysophanol 0.291 mg. CONCLUSION The methods are available with a good reproducibility and can be used to control the quality of Tongmai Jiangzhi Capsules.
出处
《中成药》
CAS
CSCD
北大核心
2005年第7期778-780,共3页
Chinese Traditional Patent Medicine
