摘要
应用温和的SDS-聚丙烯酰胺凝胶电泳,从苋菜和大豆类囊体膜中分离出10种叶绿素—蛋白复合物。按迁移率由小到大的顺序,命名为CP1a,CP1b,CP1,LHCP^4,LHCP^5,LHCP^1,LHCP^2,CPa,LHCP^3和CPx。其中,CP1b,LHCP^4,LHCP^5和CPx属于新增加的复合物,而CPx还未见报道。经鉴定表明,CPx可能就是光系统I的捕光叶绿素—蛋白LHC-I。两种植物叶绿素蛋白复合物的类型相似,与大豆相比,苋菜有更多的叶绿素与光系统Ⅰ和光系统Ⅱ反应中心叶绿素蛋白复合物结合,存在于捕光叶绿素a/b—蛋白中的叶绿素相对较少。同时还发现,苋菜捕光叶绿素a/b—蛋白复合物的多肽组成比大豆更复杂。两种植物间相应各复合的光谱性质、P—700含量和氨基酸组成无明显差异。
By using mild SDS-polyacrylamide gel electrophoresis, amaranth and soybean thylakoid membranes 10 chlorophyll-protein complexes could be resolved. According to their mobility. these complexes were respectively named CP_(1a), CP_(1b), GP_1, LHCP^4, LHCP^5, LHCP^1 LHCP^2, CPa, LHCP^3 anb CPx. CP(?), LHCP^4, LHCP^5 and CPx were new complexes found in this experiment. CPx, which was never reported in previous literature, was characterized as the light-harvesting chlorophyll a/b-protein of photo- system Ⅰ. LHC-Ⅰ. In comparison with soybean, amaranth had more chl- orophyll associated with the photosystem Ⅰ and photosystem Ⅱ reaction center complexes and less with light-harvesting chlorophyll-protein c(?)- mplexes (LHCP) though these two plants had similar pattern of chlorp- hyll-protein complexes. Moreover, it was found that polypeptide comp- ositions of amaranth LHCP were more complicated than those of soyb- ean LHCP. Spectral properties, P_(?) contents and amino acid compos- itions between amaranth and soybean chlorophyll-protein complexes had no remarkable differences.
出处
《西南农业大学学报（自然科学版）》
CSCD
1989年第2期194-201,共8页
Journal of Southwest Agricultural University
关键词
苋菜
大豆
叶绿素
蛋白复合物
Amaranthus tricolor
Glycine max
choroplast
protein
/complexes.
