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牙鲆病原秦皇岛弧菌主要生物学性状研究 被引量:2

STUDIES ON BIOLOGICAL PROPERTIES OF VIBRIO QINHUANGDAORA SP.NOV.ISOLATED FROM DISEASED FLOUNDER PARALICHTHYS OLIVACEUS
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摘要 从秦皇岛某海水养殖场牙鲆(ParalichthysolivaceusL.)细菌性败血感染症的病例中,分离到了相应的病原细菌。经对12株纯培养菌进行形态特征、培养特性、理化特性等方面较系统的表观分类学指征及代表菌株DNA中G+Cmol%的测定,表明为弧菌属(VibrioPacini1854)细菌的一个新种,并定名为秦皇岛弧菌(Vibrioqinhuangdaorasp.nov.)。同时对该菌的血清型进行了检定,表明12株菌具有同种的K抗原和同种的O抗原(血清同源);另外,经以37种抗菌类药物做敏感性测定,结果显示对供试的头孢唑啉等32种药物敏感、对青霉素G等5种耐药,在不同菌株间无明显的敏感与耐药差异。 Pathological bacteria were isolated from diseased flounder sufferd bacterial septicaemic infection in Qinhuangdao.Identification of morphological, culturing, physiological and biochemical characteristics to 12 pure cultures and detection of the mol%G+C ratio of the DNA to representative strain, showed that the pathogenic bacteria belonged to a new species of Vibrio(pacini 1854)and were designated as Vibrio qinhuangdaora sp.nov.Simultaneously, we conducted examinations on the homology of serum,the results showed that the tested 12 strains have the same O-antigen and the same K-antigen.In addition, the susceptibilities of isolates to 37 antimicrobial agents were detected, the results showed that all the strains were sensitive to cefazolin et al.,were resistant to penicillin G et al.,and there isn’t obvious discrepancy of sensitivity and resistance between strains.
出处 《水生生物学报》 CAS CSCD 北大核心 2005年第4期366-371,共6页 Acta Hydrobiologica Sinica
基金 河北省科技厅科技攻关项目(02220502D)资助
关键词 牙鲆 秦皇岛弧菌 生物学性状 血清型 耐药性 药敏试验 Paralichthys olivaceus Vibrio qinhuangdaora sp.nov Biological properties
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