摘要
目的:研究人野生型DNA聚合酶β(wtDNApolβ)在NIH3T3细胞内的定位。方法:将携带人wtDNApolβ的真核绿色荧光表达载体pEGFP-C3-polβ,用脂质体介导的方法转染NIH3T3细胞株,用荧光倒置显微镜观察转染细胞,观察wtpEGFP-C3-polβ融合基因表达。以转染空质粒pEGFP-C3的NIH3T3细胞为对照。结果:转染细胞均有GFP表达,说明转染成功。转染pEGFP-C3-polβ的细胞胞核荧光强度较胞浆强,而空质粒转染细胞胞浆胞核荧光强度无差别。结论:wtDNApolβ在NIH3T3细胞中定位于胞核内。
Aim: To locate the wide type DNA polymerase β(polβ) in the NIH3T3 cell line.Methods: The enhanced green fluorescent protein (GFP) eukaryotic expression vector pEGFP GAAB2 C3-polβ carrying wild type DNA polyβ gene was transfected into NIH3T3 cell line using the method of lipofeciamine.The location of DNA polβ gene-encoded protein in cells was observed under convert fluorescent microscope.NIH3T3 cells transfected with pEGFP-C3 were used as control.Results: The transfected cells all had GFP expression,which proved that the transfection was successful.The wide type DNA polβ gene GAAB2 encoded protein located mainly in the nuclei of DNA polβ gene transfected NIH3T3 cells and the GFP expression in nuclei was stronger than that in cytoplast;whereas the GFP expression had no difference between cytoplast and nuclei in pEGFP-C3 transfected NIH3T3 cells.Conclusion: Wide type DNA polβ gene-encoded protein locates in the nuclei of NIH3T3 cell line.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2005年第4期596-597,共2页
Journal of Zhengzhou University(Medical Sciences)
基金
*国家自然科学基金资助项目39870287
教育部科学技术研究重点项目02088
