摘要
将逆转录病毒载体（ｐＮ２－ＬａｃＺ）或ｐＮ２－ＣＭＶ－ＰｒｏＵＫ质粒，直接导入Ｗｉｓｔａｒ大鼠股四头肌、心肌或动脉管内壁，证明外源性基因可以在上述组织中表达出相应的蛋白质。外源基因在注射入股四头肌后７天表达水平最高，约可持续１～３个月以上。将带有外源基因的医用缝合线，缝合于肌肉组织中，亦可于肌肉组织中表达出相应的蛋白质，其表达效率远高于直接注射法。
N2-LacZ or pN2-CMV-ProUK plasmid DNAwas separately injected into rat
quadricep muscles andβ-galactosidase or Pro-UK was detected after 2 days.The expression of
foreign genes lasted for at least 3months. PN2-LacZ plasmid was injected into the leftventricular
wall of the rat, and the expression of LacZgene was more efficient than in the quadricep muscle.
pN2-LacZ was also introduced into the rat arterialwall by balloon catheter. A new method was
alsofound to improve the efficiency of foreign gene expres-sion in muscle by stitching medical
stitch containingplasmid DNA onto the quadricep muscle.
出处
《中华医学杂志》
CAS
CSCD
北大核心
1994年第3期158-160,T019,共4页
National Medical Journal of China
基金
"863"资助
关键词
基因治疗
心脏血管疾病
Gene therapy
Cardiovasculardiseases
