摘要
以兔抗增强子结合蛋白（ＣＣＡＡＴ／ＥｎｈａｎｃｅｒＢｉｎｄｉｎｇＰｒｏｔｅｉｎ，Ｃ／ＥＢＰ）多肽抗体，用ＡＢＣ法对１８例人正常肝组织、５例新生儿肝组织及７９例肝细胞肝癌（其中４０例带有癌旁肝组织）中Ｃ／ＥＢＰ进行免疫组化定位，同时运用Ｃ／ＥＢＰｃＤＮＡ探针对３例人正常肝组织、１例新生儿肝组织、１０例肝细胞肝癌（其中８例含配对的癌旁肝组织）进行Ｎｏｒｔｈｅｒｎ杂交分析。免疫组化结果显示，Ｃ／ＥＢＰ弥漫分布于分化成熟的肝细胞浆及肝细胞核中（胞浆含量更丰富），在肝癌分化差（或分化低）的组织细胞中含量低或检测不到，并和癌的分级存在一定相关性。所有增生的胆管上皮细胞也呈阳性。Ｃ／ＥＢＰｍＲＮＡ丰度（表达量）和免疫组化结果基本相符。以上结果进一步证实Ｃ／ＥＢＰ在维持肝细胞分化状态中担负重要作用。
/EBP is a sequenee specific DNA binding protein. In order to identify its distribution,localization and function in liver specimens from 18 normal adult,5 neonates and 79 hepatocellular carcinoma patients(40 cases associated with surrounding nontumorous tissues),immunocytochemical studies(ABC method)were performed by using anti C/EBP polypeptide antibodies 1103#,425#. Northem blot using synthesized C / EBP cDNA probe in liver tissues of 3 normal adult,one neonate and 10 hepatocellular carcinoma tissues(8 cases were associated with surrounding nontumorous tissues)were also studied.The results of immunocytochemical staining showed that C / EBP was diffusely distributed n nuclei and cytoplasm of differentiated liver cells and very low or undetectable in liver cancer cells.The expression of C/ EBP was in proportion to the degree of tumor cell differentiation and was much less than in the nontumorous surrounding tissues.C/EBP positive staining has also been found in the regen- erating epithelial cells of bile ductules.Northern blot examination matched closely with the immunocytochemical examination. The results suggest that C / EBP may play an important role in extablishing and maintaining the differentiation of liver cells and may exert an inhibiting effect against transformation of liver cells and proliferation of neoplastic tissue.
出处
《中华病理学杂志》
CSCD
北大核心
1994年第3期148-150,T030,共3页
Chinese Journal of Pathology
关键词
肝肿瘤
免疫组织化学
杂交
MRNA
Liver neoplasms Hyperplasia Immunohistochemistry Hybridization
