摘要
目的研究电刺激大鼠小脑顶核对缺血再灌注损伤时视网膜细胞形态和细胞膜Na+K+ATP酶功能的影响,及其对不同损伤程度的疗效。方法45只大鼠随机分为3组:损伤组、治疗组、假手术组。缺血再灌注损伤通过结扎大鼠视网膜血管和视神经不同时间(30、60、90min),再恢复血液灌注6h制成,其中治疗组于再灌注时用脑循环功能治疗仪刺激大鼠小脑顶核1h。用图像分析仪定量视网膜形态学改变,ATP酶试剂盒检测细胞Na+K+ATP酶活性,并通过透射电镜观察视网膜超微结构的变化。结果图像分析发现缺血再灌注损伤可使视网膜水肿,表现为厚度增加,以内视网膜为著,同时可使视网膜细胞Na+K+ATP酶活性显著降低,缺血时间越长降低越明显,与假手术组相比差异有显著性意义。而电刺激大鼠小脑顶核可明显减轻这一改变,但损伤90min时治疗组和损伤组比较差异无显著性意义。缺血再灌注可使节细胞肿胀,线粒体肿胀空泡化,内质网扩张,染色质分布不均匀,可见炎性细胞浸润,随着缺血时间延长损伤加重可有节细胞膜破裂,细胞质流失,内界膜破裂,电刺激小脑顶核可明显减轻这一病理变化。结论电刺激大鼠小脑顶核可显著改善急性缺血再灌注损伤时视网膜水肿,可显著减轻细胞损伤和恢复细胞Na+K+ATP酶功能。损伤30、60min情况下其效果显著,而损伤90min则效果较差。
Objective To study the effect of electrical stimulating cerebellar fastigial nucleus on morphology and the activities of Na^+-K^+-ATPase of cell membrane in ischemia-reperfusion injury in rat retina.Methods Forty-five rats were devided randomly into 3 groups as follows: group 1 (ischemia-reperfusion), group 2 (electrical stimulation), group 3 (sham-operated). Except group 3, the duration ischemia was achieved by ligating vessels and optic nerve for different time (30 min, 60 min, 90 min). Retinas were harvested after reperfusion for 6 h. In group 2, electrical stimulation was executed during reperfusion. Morphology changes were observed by HPIAS-1000 auto-medical-imagined computer analyzer. ATPase activities in retinal cells were measured by ATPase Kit. Under electron transmission microscopy, the changes in the retinal ultrastructure were observed.Results When ischemia time was less than 90 min, the thicknesse of inner retina was increased significantly, while the activities of Na^+-K^+-ATPase were decreased significantly in group 1 as compared with those in group 2. When ischemia time was over 90 min, there was no significant difference in the thickness and activity of Na^+-K^+-ATPase between two groups. The morphological changes of cell edema, plasmosome swelling, endoplasmis reticulum expansion and inflammatory corpuscle infiltrating were deserved under electron transmission microscopy in group 1. These morphological changes were alleviated in group 2.Conclusion Electrical stimulating cerebellar fastigial nucleus can obviously improve the retinal edema caused by acute ischemia-reperfusion injury, significantly alleviate the damage to cells and recover the function of Na^+-K^+-ATPase. The effects of electrical stimulation was significant when the ischemia time was less than 90 min, while the effects were worse when the ischemia time was over 90 min.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2005年第3期363-366,共4页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
