摘要
利用旋转回归法研究里氏木霉RutC-30发酵生产纤维素酶的2个重要因素:微晶纤维素粉(Avicel)、麸皮对滤纸酶活的影响,并拟合出回归方程。经回归分析表明,培养基中Avicel、麸皮的含量及其配比对滤纸酶活有显著影响。通过岭脊分析寻优得出:Avicel最佳浓度为1·34%、麸皮最佳浓度为3·35%,在此优化条件下滤纸酶活可达6·51IU/ml。用30L发酵罐进行放大试验,滤纸酶活可达10·84IU/ml,CM-Case达到449·57IU/ml。
With the quadratic rotary design,two important factors-Avicel and wheat bran which plays an important part in cellulase fermentation by Trichoderma reesei Rut C-30,and.Regression analysis showed that the contents and the proportion of Avicel and wheat bran in the medium influenced the FPA significantly.Through ridge analysis,the optimum Avicel and wheat bran contents are 1.34% and 3.35% respectively.In this optimized condition,the FPA can reach 6.51 IU/ml。10.84 IU/mL of FPA and 449.57 IU/ml of CMCase were obtained when the process was scaled up to 30 L fermentor.
出处
《食品与机械》
CSCD
北大核心
2005年第3期29-31,共3页
Food and Machinery
关键词
正交旋转组合
纤维素酶
发酵
碳源
岭脊分析
Cellulase
Trichoderma reesei
Ridge analysis
Medium optimization
