摘要
目的:将睫状神经营养因子和神经生长因子应用于去神经带血管移植肌,探讨2种因子在神经再生过程中的作用。方法:实验于2001-03/2002-03在解放军总医院实验动物中心完成。选用54只SD大鼠,随机分为3组,每组18只,分别为睫状神经营养因子组、神经生长因子组和对照组。①各组在切除腓总神经后,将腓骨长肌远端切断并切除肌膜后植入同侧腓肠肌。从手术当天开始,睫状神经营养因子组每天向移植肌注射睫状神经营养因子0.05μg/每后肢,神经生长因子组注射神经生长因子0.5μg/每后肢,对照组注射生理盐水,共7d。②术后30和60及90d取移植肌,通过乙酰胆碱酯酶染色观察运动终板。③采用链霉素抗生物素蛋白-过氧化物酶连接法检测移植体肌组织中胶质细胞源性神经生长因子抗体,观察染色强度以反映神经再生情况。④电子显微镜下观察肌细胞形态,核仁、染色质、糖原颗粒、Z线结构及神经纤维及髓鞘情况以反映失神经肌肉受损程度。⑤光学显微镜下观察肌纤维、神经纤维及髓鞘变化以反映失神经肌肉受损程度及神经再生情况。结果:大鼠54只均进入结果分析。①神经生长因子组和睫状神经营养因子组在术后30和60及90d时的肌细胞形态,核仁清晰度,可见染色质丰富程度方面优于同期对照组,并且糖原颗粒多,Z线更清晰。神经再生更明显。②神经生长因子和睫状神经营养因子组有更多的运动终板。③神经生长因子组和睫状神经营养因子组胶质细胞源性神经营养因子抗体染色强度大于对照组。神经生长因子组和睫状神经营养因子组相比,神经生长因子组的促神经再生作用更明显。结论:①神经生长因子和睫状神经营养因子可以促进自体去神经带血管移植肌的神经再生和减轻失神经肌肉的损害。②上述2种因子均可促进移植肌神经再生,神经生长因子作用更强。
AIM:To apply ciliary neurotrophic factor and nerve growth factor in denervated vascularized skeletal muscle, and investigate the role of these two factors in the process of neurotization.METHODS:The study was carried out in the Experimental Animal Center of the General Hospital of Chinese PLA between March 2001 and March 2002. Fiftyfour SD rats were randomly divided into 3 groups with 18 rats in each group: ciliary neurotrophic factor group,nerve growth factor group and control group.①After resection of common peroneal nerve,the distal peroneus longus muscle was broken and sarcolemma was resected,and then gastrocnemius muscle of the same side was implanted. From the day of surgery, ciliary neurotrophic factor (0.05 μg per posterior limb) was injected into the grafted muscle every day in the ciliary neurotrophic factor group, rats in the nerve growth factor group were injected with nerve growth factor(0.05 μg per posterior limb) and those in the control group were injected with saline, they were treated for 7 days.②The grafted muscles were taken 30,60 and 90 days postoperation,and motor end plate was observed with acetylcholinesterase staining.③The glial cell nerve growth factor antibody in the tissue of grafted muscle was detected with the method of avidinperoidase connection;The stained intensity was observed to reflect the condition of neurotization.④The form of myocyte,nucleolus,chromatin,glycogen granule, Z linear structure, nerve fiber and myelin sheath conditions were observed under electron microscope to reflect the damaged severity of denervated muscles.⑤Muscle fiber,nerve fiber and myelin sheath changes were observed under light microscope to reflecte the damaged severity of denervated muscles and conditions of neurotization.RESULTS:All the 54 rats were involved in the analysis of results.①The form of myocyte, acuity of nucleolus and plenty degree of chromatin 30,60 and 90 days postoperation in the nerve growth factor group and ciliary neurotrophic factor group were superior to those in the control group, and there were more glycogen granule,more clear Z line and more obvious neurotization.②There were more motor end plates in the nerve growth factor group and ciliary neurotrophic factor group.③The stained intensity of glial cell nerve growth factor antibody was greater in the nerve growth factor group and ciliary neurotrophic factor group than in the control group.The promotive role of neurotization was more obvious in the nerve growth factor group than in the ciliary neurotrophic factor group.CONCLUSION:①Nerve growth factor and ciliary neurotrophic factor can promote the muscular neurotization of autologous denervated vascularized skeletal muscle and relieve the damage of denervated muscle.②Both of the above two factors can promote the neurotization of grafted muscle,and the role of nerve growth factor is more effective.
出处
《中国临床康复》
CSCD
北大核心
2005年第21期108-109,共2页
Chinese Journal of Clinical Rehabilitation
