摘要
目的探讨环氧合酶2(cyclooxygenase-2,COX-2)和血管内皮生长因子(vascularendothelial growthfactor,VEGF)在人胃癌组织中表达及其相关性。方法应用免疫组织化学SABC法检测53例人胃癌组织中COX-2、VEGF和CD34的表达,并以40例正常胃粘膜标本作为对照。对CD34阳性血管进行微血管密度(microvesseldensity,MVD)计数。对COX-2和VEGF的表达采用半定量计分法判定,并结合临床资料进行统计学分析。结果53例人胃癌组织中,COX-2表达阳性者44例,阳性率为83.0%;VEGF表达阳性者45例,阳性率为84.9%。COX-2表达与VEGF表达相关显著(P<0.05)。并且,COX-2和VEGF的表达与TNM分期(P<0.05,P<0.05)、淋巴结转移(P<0.01,P<0.05)和远处转移(P<0.01,P<0.05)相关。COX-2/VEGF同高表达组中MVD值(79.5±25.8)高于COX-2/VEGF同低表达组中的MVD值(45.0±13.9),差异非常显著(P<0.01)。结论胃癌组织中COX-2与VEGF共表达,并相互协同促进肿瘤血管生成和转移。
Objective To study the expressions of cyclooxygenase-2 (COX-2) and vascular endothelial growth factor (VEGF) in gastric cancer and to explore their correlation in gastric carcinoma. Methods The expressions of COX-2 and VEGF were studied by immunohistochemistry in 53 gastric cancer specimens and 40 normal tissues as control. The microvessel density (MVD) was assayed by CD34 positive blood vessels counting. Results Expressions of COX-2 and VEGF in gastric cancer tissues were 83.0% and 84.9% respectively. The expression of COX-2 was correlated significantly with VEGF expression (P< 0.05). Expressions of COX-2 and VEGF were highly correlated with TNM stage(P< 0.05,P< 0.05) ,lymph node metastasis(P< 0.01, P< 0.05) and distant metastasis (P< 0.01, P< 0.05). The MVD value was much higher in COX-2/VEGF double strong expression group than that in COX-2/VEGF double weak expression group (P< 0.01). Conclusion COX-2 overexpression is remarkably correlated with that of VEGF and they may play an important role in tumor angiogenesis and metastasis of human gastric cancer.
出处
《现代肿瘤医学》
CAS
2005年第3期294-297,共4页
Journal of Modern Oncology
基金
国家自然科学基金资助项目(编号30130260)
