摘要
采用电镜观察和聚丙烯酰胺垂直板电泳法,研究了GB对低温胁迫下黄瓜幼苗叶绿体超微结构和SOD、POD同工酶的影响。结果表明,低温胁迫下,GB可使黄瓜幼苗叶绿体膜结构保持完好,而对照的叶绿体基粒破坏严重;与对照相比,GB处理对SOD、POD同工酶谱带数目均无影响,但POD同工酶的P2(Rf0.24)、P3(Rf0.30)、P4(Rf0.36)酶带的活性增加,SOD同工酶的S1(Rf0.14)、S5(Rf0.58)酶带活性较高,保持了黄瓜幼苗体内相对较高的抗氧化酶活性。GB可保护膜的完整性和稳定性,从而提高黄瓜幼苗的抗冷性。
The effects of GB on the chloroplast ultrastructure and isozymes of SOD and POD of cucumber seedlings under cold stress were studied by electron microscope and PAGE. The results showed that the chloroplast membrane structure of cucumber seedlings treated with GB was not damaged, but that of the control was damaged. Compare with the control,the band numbers of SOD and POD isozymes were not changed by GB, but the activities of bands[P2(Rf 0.24),P3(Rf 0.30),P4(Rf 0.36)]of POD isozyme and some bands of SOD isozyme were increased. It showed that GB could protect the integrity and stability of the membrane, and enhance the cold resistance of cucumber seedling.
出处
《生物技术》
CAS
CSCD
2005年第3期25-27,共3页
Biotechnology
