摘要
目的探讨人参皂甙Rb1对离体兔肺的保护作用及其机制。方法应用离体肺缺血再灌注模型,将20只健康家兔随机分为两组,实验组以人参皂甙Rb1(0.08mg/ml)加入含PGE1的低钾右旋糖酐(LPD)液进行肺灌注及保存,对照组则以含PGE1的LPD液灌注及保存,4℃保存12h后再灌注60min。灌注过程中检测肺血管阻力(PVR)、肺静脉血氧分压(PO2);灌注完毕测定肺组织含水量(LW)、一氧化氮(NO)、超氧化物歧化酶(SOD)、丙二醛(MDA)含量;应用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法(TUNEL)对肺凋亡细胞进行检测并观察肺组织超微结构变化。结果实验组PVR、LW较对照组明显降低(P<0.05),PO2明显升高(P<0·01);实验组肺组织NO、SOD含量明显高于对照组(P<0.05),MDA含量则较对照组低(P<0·05),其细胞凋亡指数(AI)亦较对照组显著减少(P<0·05),超微结构变化明显较对照组轻。结论人参皂甙Rb1能减轻肺缺血再灌注损伤,增加肺的保存效果,其作用机制与清除氧自由基、抗氧化、抗细胞凋亡有关。
Objective To explore the protective effect of ginsenoside on lung reperfusion injury and lung preservation in a isolated lung reperfusion model. Methods Twenty rabbits were randomly divided into two groups. In control group, the lungs were flushed and preserved with low potassium dextran solution(LPD) containing PGE1, and in treated group, 0.08 mg/ml ginsenoside were added. The lungs were preserved at 4 ℃ for 12 h and reperfused with whole blood for pulmonary functional assessment. During reperfusion, PVR and PO2 were measured. After reperfusion, the content of lung water, nitric oxide(NO), superoxide dismutase(SOD), malondialdehyde (MDA) were determined. The lung apoptotic cells were stained by the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling(TUNEL) technique. Light microscopic and ultrastructural changes were also examined. Results The PVR and LW were significantly lower in treated group than in control group. The PO2 was much higher in treated group than in control group. The content of NO and SOD in treated group were significantly higher than in control group but the content of MDA was adverse. The apoptosis index(AI) of lung cells was markedly reduced in treated group than in control group. The morphologic and ultrastructural damages in treated group were significantly milder than in control group. Conclusion Ginsenoside can attenuate the lung ischemic reperfusion injury, and improve the protective effect on the preservative lung.
出处
《山西医科大学学报》
CAS
2005年第3期310-312,共3页
Journal of Shanxi Medical University
基金
深圳市科技基金资助项目(200204091)
关键词
肺
再灌注损伤
人参皂甙
兔
lung
reperfusion injury
ginsenoside
rabbits
