摘要
利用铝形态分布与环境pH的相关性,通过改变染毒液pH条件,研究了不同浓度活性铝对小麦根生长、蛋白质含量及酸性磷酸酶活性的影响,并探讨了不同形态活性铝植物毒性的差异.本实验染毒液中总铝浓度设置为0(CK)、25(T1)和75μmol·L-1(T2)3组,各组pH分别调至4.0、4.5、5.0和5.5.结果表明,微量Ala与Alb对小麦根生长均具有抑制作用.但随染毒液中活性铝组分的改变,小麦根蛋白质含量和酸性磷酸酶活性显现相反变化趋势:T1和T2组在pH4.0,活性铝主要成分为Ala时(Ala浓度高于活性铝浓度的90%),小麦根细胞蛋白质含量显著下降,酸性磷酸酶活性显著上升;T1和T2组在pH5.0,Ala浓度降低至与Alb浓度接近,且Ala和Alb浓度均低于10μmol·L-1时,根细胞蛋白质含量显著上升,酸性磷酸酶活性显著下降.
Aluminum speciation is recognized to associate with environmental pH.In this paper,the effects of aluminum speciation (Al_a,Al_b) on the growth,protein content,acid phosphatase (AP) activity of wheat root were studied by changing the pH of aluminum solutions.Two concentrations of total aluminum,25 μmol·L^(-1) (T_1) and 75 μmol·L^(-1) (T_2),were set up in the test solutions,and four levels of pH,4.0,4.5,5.0 and 5.5,were regulated for each solution.The results indicated that the growth of wheat root in T_1 and T_2 was strongly inhibited by low doses of Al_a and Al_b,while the changing trend of protein content and AP activity varied when exposed to different composition of active Al.When the Al_a concentration was above 90% of the active Al concentration,the protein content was reduced while the AP activity was enhanced by Al at pH 4.0,but it was reverse at pH 5.0 when the concentration of Al_a and Al_b was below 10 μmol·L^(-1),respectively.
出处
《应用生态学报》
CAS
CSCD
北大核心
2005年第6期1043-1046,共4页
Chinese Journal of Applied Ecology
基金
国家自然科学基金资助项目(39570150).
关键词
活性铝
小麦
蛋白质
酸性磷酸酶
Wheat, Aluminum speciation, Acid phosphatase (AP), Protein.
