摘要
采用逆转录聚合酶链反应检测急性早幼粒细胞白血病缓解患者的残留早幼粒白血病－α维甲酸受体融合基因（ＰＭＬ－ＲＡＲα）。缓解期在３年之内的１０例中９例阳性，１例阴性，缓解期在３年以上者７例，４例阴性，３例阳性，两组阳性率相差显著，Ｐ＜０．０５。在ＰＭＬ－ＲＡＲα表达阳性的患者中已有３例复发，１例复发趋势，５例ＰＭＬ－ＲＡＲα阴性患者已随访１１±４月均无复发迹象。对阴性患者放松治疗，对阳性患者继续治疗，４例原已停止治疗的患者结果阳性，继续治疗后目前随访１２个月（中数），尚无复发证据。
n this study nesting polymerase chain reaction ofreverse transcription was employed to investigate thefusion transcripts of PML-RARα in 17 remissionpatients with acute promyelocytic leukemia. Afterachieving complete remission (CR) by the induction ofretinoic acid, the transcripts of the fusion gene werepositive in 9 and negative in 1 out of the 10 patientswhose CR durations were less than 3 years. However,among 7 cases with CR duration over 3 years, 4 caseswere negative and 3 positive. The difference betweenthe two groups was statistically significant(P<0. 025).Among all the positive patients, relapse was proved in 3cases and relapse tendancy in one patient. No evidenceof relapse was found in 5 negative patients at 11± 4months' follow-up. Further treatment was thereforegiven to 4 positive patients who already ceased therapy.They all remained in CR status in the following 12(median)months' observation. So we considered thatthe PML-RARα gene might represent the minimalresidual disease of APL. Its fusion transcripts wereclosely associated with relapse and the detection of itcould guide effectively clinical continuation treatment.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
1994年第7期359-361,共3页
Chinese Journal of Hematology
关键词
聚合酶链反应
微量残留
白血病
Polymerase chain reaction Minimalresidual disease PML-RARα fusedgene
