摘要
采用逆转录－筑巢式聚合酶链反应（ｎｅｓｔｅｄＰＣＲ）技术检测３３例不同临床阶段的慢性粒细胞白血病（ＣＭｕ及１８例急性淋巴细胞白血病（ＡＬＬ）患者的ｂｃｒ／ａｂｌｍＲＮＡ。结果：３３例ＣＭＬ中３１例阳性，其中２１例表达ｂｃｒｅｘｏｎ３／ａｂｌｅｘｏｎ２ｍＲＮＡ，７例表达ｂｃｒｅｘｏｎ２／ａｂｌｅｘｏｎ２ｍＲＮＡ，２例同时表达这两种ｍＲＮＡ。在１８例ＡＬＬ中阳性６例，其中４例表达ｈｒｅｘｏｎ３／ａｂｌｅｘｏｎ２ｍＲＮＡ，２例表达ｂｃｒｅｘｏｎ３／ａｂｌｅｘｏｎ２＋ｂｃｒｅｘｏｎｌ／ａｂｌｅｘｏｎ２ｍＲＮＡ。本实验的敏感度达１０￣（－６）～１０￣（－７）水平，有助于临床上白血病微量残留病的早期诊断。
e detected bcr/abl fusion gene with reverse tran-scription-nested polymerase chain reaction(nested PCR)assay in 33 patients with chronic myeloid leukemia(CML) in different clinical stages and 18 patients withacute lymphoblastic leukemia(ALL).Thirty one out of33 patients with CML were positive.Among 31 positivecases,21 patients showed bcr exon 3/abl exon 2 mR-NA,7 patients showed ber exon 2/abl exon 2 mRNA ,and two types of mRNA junction were detected in 2 pa-tients.Six out of 18 patients with ALL were positive.4patients showed ber exon 3/abl exon 2 mRNA,2 pa-tients showed both types of mRNA junction. The sensi-tivity of this method was 10￣(-6)～10￣(-7). With this sensi-tive nested PCR assay,we could detect the minimalresidual disease at a very early stage.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
1994年第5期227-229,共3页
Chinese Journal of Hematology
基金
国家自然科学基金
广东省青年科学基金
关键词
聚合酶链反应
白血病
髓细胞性
Nested polymerase chain reactionbcr/abl fusion gene Chronic myeloidleukemia Acute lymphoblasticleukemia
