摘要
用氩离子激光（波长５１４ｎｍ）照射，部花青（ＭＣ５４０）介导的光敏作用对白血病细胞系ＨＬ－６０、Ｋ５６２，急性髓细胞白血病原代祖细胞（ＣＦＵ－Ｌ）以及正常骨髓ＣＦＵ－ＧＭ的杀伤敏感性作了比较；用电镜观察处理前后Ｋ５６２细胞的超微结构变化。单纯激光照射或仅有ＭＣ５４０而不照光，对ＨＬ－６０细胞集落产率及ＣＦＵ－ＧＭ产率无明显影响。当ＭＣ５４０浓度为２５μｇ／ｍｌ、激光照射能量密度为７９．５１／ｃｍ￣２的条件处理，ＨＬ－６０集落产率下降２．８个对数级、ＣＦＵ－Ｌ减少２．２个对数级，而骨贿ＣＦＵ－ＧＭ尚能保留６４．０±７．０％；含１％Ｋ５６２细胞的模拟白血病骨贿经同样处理，Ｋ５６２集落产率能下降１．３６对数级，而ＣＦＵ－ＧＭ尚存７４．０±１９．０％。电镜观察发现处理后的Ｋ５６２细胞体积增大，肿胀，胞膜洞孔样破坏。结果提示，氩离子激光照射，ＭＣ５４０介导的光敏作用能选择性杀伤白血病细胞系，原代白血病祖细胞同样敏感。其对细胞的杀伤作用可能主要因直接造成胞膜破坏。该方法将可能成为一种有前途的自体骨髓移植物体外净化手段。
he purpose of this study was to determine the sensi-tivity to argon ion laser-induced merocyanine 540(MC540)- mediated photolysis of leukemic cells and nor-mal marrow CFU-GM. HL-60 cells ,marrow cells of acute myelogenous leukemia ( AML),simulatedleukemic marrow cells with 1%occulted K562 cells,andnormal marrow cells were incubated respectively withMC540(25μg/ml) in the presence of 15%newborn calfserum and simutaneously exposed to 79.5J/cm￣2 laser ir-radiation. The results showed that 2. 8 log of HL-60 cellcolonies and 2.2 log of CFU-L were killed respectively,whereas 64.0±7.0%of CFU-GM survived. In simulat-ed leukemic marrow,K562 colonies were reduced by1. 36 log while 74.0±19.0%of CFU-GM remained.There was no significant reduction in HL-60 colonies orCFU-GM when HL- 60 cells or marrow cells were ex-posed to laser light alone or incubated with MC540 inthe dark.A damaged membrane with various size holeswas observed in K5 6 2 cells after MC540-mediated pho-tosensitization.The results indicated that MC540-medi-ated photosensitization has selective cytotoxic effect onleukemic cells and it might be promising for purging au-tologous bone marrow grafts in vitro.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
1994年第6期304-307,共4页
Chinese Journal of Hematology
关键词
部花青
光敏作用
骨髓净化
白血病
Merocyanine 540(MC540)Photo-sensitization Bone marrow purging
