摘要
目的:探讨Bcl2和Bax蛋白在大鼠局灶性脑缺血预处理脑组织中的表达及意义。方法:线栓法阻断SD大鼠大脑中动脉建立脑缺血预处理及脑缺血模型。TTC染色法测量脑梗死体积,免疫组织化学检测前脑皮质、基底核Bcl2和Bax蛋白的表达。结果:与缺血组相比,预处理缺血组脑梗死体积明显减小(P<0.01),Bcl2蛋白表达细胞数明显增加(P<0.01),Bax蛋白表达减少(P<0.05)。结论:缺血预处理诱导Bcl2蛋白表达增加和Bax蛋白表达下降可能是缺血预处理诱导抗凋亡、产生脑保护机制的原因之一。
Objective: To study expression and significance of anti-apoptosis proteins Bcl-2 and Bax in preconditioning focal cerebral ischemia tissue of rats. Methods: Suture-occluded method was used to block middle cerebral artery, and preconditioning cerebral ischemia and cerebral ischemia model of SD rats was made. The volume of cerebral infarction was detected with TTC staining. The expression of Bcl-2 and Bax in front cerebral cortices and basal nuclei was observed by using immunohistochemistry method. Results: Compared with ischemia group, the volume of cerebral infarction reduced significantly (P<0.01). Cells expressed Bcl-2 protein increased (P<0.01), and that expressed Bax protein decreased (P<0.05) in preconditioning ischemia group. Conclusion: Preconditioning ischemia could induce increase of Bcl-2 and decrease of Bax, which may be one of the mechanisms in anti-apoptosis and protection of cerebral cells.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2005年第3期227-228,共2页
Journal of China Medical University
