摘要
以丙酮萃取甲壳素加工废水的絮凝物,再经石油醚萃取丙酮液得虾青素粗提品.为提高游离虾青素含量,研究了从粗提品中提纯虾青素的工艺,确定了以Na2CO3水溶液洗涤除杂质的方法.Na2CO3溶液浓度为0.025g/mL,其用量为石油醚萃取液体积的1.2倍,25℃时振摇6min.以色素保留率和杂质的去除量作为提纯工艺的评价指标.由10g絮凝物得到3.3g粗提品,以最佳工艺条件洗涤除杂,最后得0.22g提纯品.提纯后游离虾青素含量经HPLC测定达6.94%,与未提纯样品相比较,效果较好.
<Abstrcat> The coarse product of astaxanthin which was prepared from the flocculate of the process wastewater of chitin was extracted by acetone, then extracted again by petroleum ether. In order to increase the content of free astaxanthin, a technology was designed to purify astaxanthin from the coarse product of astaxanthin. It was determinated that Sodium carbonate was choosed as edulcoration solution, the solution strength was 2.5% gramme per milliliter, the dosage of sodium carbonate is 1.2 times of the volume of petroleum ether extraction solution,the reaction temperature and time were 25 ℃ and 6 minutes. The pigment retention rate and amount of impurity removing were choosed as the evaluation index of the purification technology. 3.3 gramme coarse product was gained from 10 gramme of the flocculate of the process wastewater of chitin. Edulcorated with Optimized process conditions,finally 0.22 gramme purified astaxanthin sample was gained.The content of the free astaxanthin was 6.94% in the purified astaxanthin sample which was analysized by HPLC, the fruitage was much better than the coarse product.
出处
《浙江工业大学学报》
CAS
2005年第3期324-327,340,共5页
Journal of Zhejiang University of Technology
关键词
虾青素
洗涤
纯化
astaxanthin
lavation
purification
