摘要
目的 :观察神经再生素 (NRF)作用于离体培养的SD大鼠大脑皮层细胞过程中细胞活性、基因的表达变化 ,探讨NRF促神经生长机制。方法 :SD大鼠大脑皮层细胞分离、培养于含NRFL15培养基 ,采用RT PCR方法检测培养细胞生长相关蛋白 4 3(GAP 4 3)、神经丝蛋白 (NF L)和锌指样蛋白 (DDP2 )基因经NRF作用后基因表达变化 ,MTT法检测细胞活力。结果 :2 μg/mlNRF作用于培养大脑皮层细胞为较佳使用浓度 ;NRF作用 6～ 12h ,GAP 4 3、NF L和DDP2基因表达分别上调至峰值 2 0 0 % ,15 0 % ,135 % ;NRF作用 2 4h后 ,基因表达回复至正常值 ,而细胞活力仍上调 2 3 2 %。结论 :NRF可通过上调神经生长相关基因表达 ,提高细胞活力达到促神经生长。
AIM:To detect the variety of some neueotization relative gene expressions in cultured SD rat brain mantle cell and cell activity cocultured with nerve regeneration factor(NRF) and to explore the mechanism of NRF in promoting neuron growth.METHOD:The cell from SD rat brain mantle cocultured with NRF in L15 contrast with no NRF, quantitative analysis GAP43、DDP2、NF-L gene expression by reverse transcription-polymerase chain reaction .Cell activity analysis by MTT. RESULT:NRF 2μg/ml has the best effect on regulating some neueotization relative gene expression in cultured SD rat brain mantle cell. The gene expression of GAP43、DDP2、NF up-regulated to its peak value about 200%, 150%, 135% in SD rat brain mantle cell cocultured with NRF(2 μg/ml) 6～12 h and back to its normal value after 24 h although the cell activity was increased by 23.2% yet. CONCLUSION:NRF may up regulate some regulation factor in cultured SD rat brain mantle cell and increase cell activation to promote neuron growth.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
2003年第4期365-368,共4页
Journal of China Pharmaceutical University
基金
国家自然科学基金资助项目 (编号 3 0 0 70 2 5 5 )
江苏省自然科学基金资助项目 (编号BK2 0 0 113 2 )
关键词
神经再生素
基因表达
细胞活性
大脑皮层细胞
Nerve regeneration factor(NRF)
Gene expression
Cell activity
Brain mantle cell
