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白细胞介素-6参与肿瘤相关抗原HCA520对HEK293细胞的促增殖作用 被引量：1

Interleukin-6 is involved in the enhanced proliferation of HEK293 cells by the tumor specific antigen of HCA520
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摘要 目的:研究肿瘤相关抗原HCA520对HEK293细胞增殖的影响,获得HCA520生物学功能的初步提示。方法:构建pcDNA3 HCA520 flag真核表达载体转染HEK293细胞并建立稳定转染细胞系,利用MTT实验观察HCA520对HEK293细胞增殖速率的调节。通过RT PCR检测可能参与HCA520对HEK293细胞增殖促进作用的相关分子,并用3 (4, 5 二甲基2 噻唑) 2, 5 二苯基溴化四唑(MTT)实验进一步验证目标分子对HEK293细胞增殖的作用。结果:MTT实验结果显示HCA520显著提高HEK293细胞的增殖速率,HCA520上调白细胞介素6在HEK293细胞中的表达,后者部分参与HCA520对HEK293细胞的促增殖作用。结论:HCA520对HEK293细胞的增殖具有促进作用,并推测可能通过促进细胞恶性增殖在肿瘤发生中发挥作用。 Objective: To investigate the effect of tumor specific antigen of HCA520 on the proliferation of HEK293 cells and to obtain some functional implications for HCA520. Methods: MTT assay was performed with HEK293 stable cell lines transfected with pcDNA3-HCA520-flag construct. Cytokines probably involved in HCA520 enhanced proliferation were screened by RT-PCR, and effect of these cytokines on the HEK293 cell proliferation was further confirmed by MTT assay. Results: HCA520 significantly promoted the proliferation of HEK293 cells, which was at least partially attributed to the up-regulation of IL-6 in HEK293 cells by HCA520. Conclusion: HCA520 might accelerate tumorigenesis by promoting proliferation of cancerous cells.
作者 钱晓萍 李国栋 韩克军 陈慰峰
机构地区 北京大学基础医学院免疫学系
出处 《北京大学学报（医学版）》 CAS CSCD 北大核心 2005年第3期310-313,共4页 Journal of Peking University:Health Sciences
基金 国家自然科学基金资助(30300309)~~
关键词 HEK293细胞 HCA520 白细胞介素-6 肿瘤相关抗原 促增殖作用 细胞增殖 真核表达载体 细胞恶性增殖 生物学功能 转染细胞系 PCR检测 MTT实验 实验观察 相关分子 发挥作用 肿瘤发生 二甲基 速率 Antigens, neoplasms Cell line Cell division Cytokines Interlenkin-6
分类号 R394 [医药卫生—医学遗传学] R730.23 [医药卫生—肿瘤]
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参考文献10

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二级参考文献48

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共引文献2

同被引文献15

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北京大学学报（医学版）
2005年 第3期

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