摘要
目的:了解雌、孕激素和米非司酮对子宫内膜癌细胞生长的影响。方法: 体外培养高分化子宫内膜癌细胞Ishikawa和中分化子宫内膜癌细胞Hec 1B, 分为对照组、雌激素组、雌孕激素联合组、雌孕激素和米非司酮联合组,在24, 48, 72, 96h测定各组细胞生长趋势的变化。结果: 雌激素作用72hIshikawa细胞生长显著高于对照组,Hec 1B细胞较对照组有升高趋势,但差异无统计学意义。孕激素作用72h对Ishikawa细胞有显著抑制作用, 96h后对Hec 1B细胞有显著抑制作用。在雌、孕激素基础上加入米非司酮96h后Ishikawa细胞明显增生, 48h后Hec 1B细胞明显增生。结论:雌激素可以刺激子宫内膜癌细胞的生长。孕激素在雌激素基础上抑制内膜癌细胞的生长。米非司酮有拮抗孕激素的抑制子宫内膜癌细胞生长的作用。
Objective: To study the effects of estrogen, progestogen and mifepristone on endometrial carcinoma cell lines in vitro. Methods: The well-differentiated endometrial cancer cell line Ishikawa and moderate-differentiated endometrial cancer cell line Hec-1B were cultured in vitro. The cells were divided into four groups:control, estrogen , estrogen and progestogen , estrogen and progestogen and mifepristone, then we implanted cells in 96-well plates to search the response in distinct hormones. Results: When stimulated by estrogen for 72 hours, the growth of Ishikawa cells was significantly higher than the control, Hec-1B cells only grew higher than the control, but it was no significance in statistics. Progestogen inhibited Ishikawa cells significantly after being stimulating for 72 hours, there was the same effect on Hec-1B cells after being stimulating for 96 hours. On the base of estrogen and progestogen, we added mifepristone,cells developed after 96 hours in Ishikawa cells and cells developed after 48 hours in Hec-1B cells. Conclusion: Estrogen can cause endometrial carcinoma cell growth; progestogen inhibits the hyperplasia induced by estrogen; mifepristone antagonizes the effect of progestogen on cell growth.
出处
《北京大学学报（医学版）》
CAS
CSCD
北大核心
2005年第3期284-286,共3页
Journal of Peking University:Health Sciences
基金
国家自然科学基金(30170977)资助~~
