摘要
Ｃａ２＋的摄取和释放是成骨细胞的重要功能．我们应用４５Ｃａ放射测定技术研究原代培养成骨细胞的摄钙能力及生长激素（ＧＨ）的影响．结果表明，１５分钟时细胞含４５Ｃａ量为０分钟的２～３倍，３０分钟时继续增加．ＧＨ刺激细胞对４５Ｃａ的摄取，３０分钟时较对照组增加２．３倍，６０分钟时增加１．５倍．
Abstract Uptake and release of Ca2+are important functions of osteoblasts. In the paper we studied the uptake of calcium and influence by Growth Hormone in osteoblasts of fetal rat calvaria by liquid Scintillation spectrometry of 45Ca2+. In short-term cultures of the bone derived cells, the uptake of 45Ca2+ increased steadily. The activity of 45Ca2+ in the cells of 15 minute cultures was 2￣3 times of that in the 0 minute cultures. It continued to increase in the cells of 30 minute cultures. Exposure of the bone cells to GH at 55.3ng/ml increased the uptake of 45Ca2+ by 2.3 times in the 30 minute cultures and 1.5 times in the 60 minute cultures than those of the control.
出处
《中华核医学杂志》
CAS
CSCD
北大核心
1994年第3期173-174,共2页
Chinese Journal of Nuclear Medicine
基金
国家自然科学基金
