摘要
我国有丰富廉价的动植物油资源,但这一资源并未得到有效的利用,为了充分利用这些资源,开展了无溶剂系统脂肪酶催化猪油与辛酸酸解制备功能性脂的研究工作。脂肪酶筛选实验表明,在所选用的五种脂肪酶中,来自T .languginosa的固定化脂肪酶LiopzymeTLIM的催化效果最好。以LipozymeTLIM为催化剂,进一步研究了酶量、底物比率、反应时间、反应温度和水分添加量对猪油中辛酸插入率的影响。反应产物通过高效液相色谱法(HPLC)进行分析。研究结果表明,当脂肪酶量为2 0 % (底物重量百分比)时,猪油中辛酸插入率最高。反应时间研究表明,当反应时间达到2 4h时,辛酸的插入率最高,达到38 77mol%。当猪油与辛酸的比率为1∶2 (摩尔比)时,辛酸的插入率最高,达到30 95mol%。在4 5～6 0℃范围之内,反应温度对辛酸插入率没有明显的影响,温度高于6 0℃时,辛酸插入率降低。水分添加量为2 5 %时,辛酸插入率最高,高达35 76mol%。
China has richly and inexpensive fat and oils from animal and plants, but these resources could not get effectively utilization. In order to make the best of these resources, lipase-catalyzed acidolysis of lard with caprylic acid to produce functional lipid in solvent free system was investigated. Of the five lipases that were tested in the initial screening, immobilized lipase TL IM fromca T. languginosa resulted in the highest incorporation of capry lic acid into lard. This enzyme was further studied for the effect of enzyme load, substrate ratio, reaction time, reaction temperature and added water content on the incorporation of caprylic acid into lard. HPLC analyzed the products from the acidolysis reaction. The highest incorporation was attained at 20% enzyme load. Time course studied suggest that the incorporation of caprylic acid into lard was increased up to 38.77mol% after 24h. Desirable mole ratio of substrates was 1:2 (lard: caprylic acid), caprylic acid incorporation up to 30.95mol%. In the range of 45～60℃, temperature had no significant effect on enzyme activity and caprylic acid incorporation changed little. When temperature was above 60℃,incorporation of caprylic acid into lard was decreased. The highest incorporation of caprylic acid into lard 35.76 mol% was attained when added water content was 2.5%.
出处
《生物工程学报》
CAS
CSCD
北大核心
2005年第3期493-496,共4页
Chinese Journal of Biotechnology
关键词
脂肪酶
酸解反应
猪油
辛酸
功能性油脂
无溶剂体系
lipase, acidolysis, lard, caprylic acid, structured lipid, solvent free system
