摘要
目的研究选择性环氧合酶-2(COX-2)抑制剂罗非昔布(rofecoxib)对人胃癌细胞株SGC7901增殖的影响,并探讨其作用机制。方法采用MTT比色分析法测定人胃癌细胞株SGC7901分别在1×10-5、1×10-6、1×10-7、1×10-8、1×10-9、1×10-10molL的罗非昔布作用72h后的增殖及凋亡情况;增殖细胞核抗原(PCNA)免疫细胞化学染色观察罗非昔布对胃腺癌细胞SGC7901增殖的影响;采用免疫细胞化学染色观察1×10-5molL的罗非昔布对SGC7901细胞c-myc基因表达的影响。结果1×10-5～1×10-9molL的罗非昔布对SGC7901细胞的生长均有抑制作用,以1×10-5molL浓度的抑制作用最为显著,其抑制率为35.46%;1×10-5molL罗非昔布作用后SGC7901的PCNA表达明显降低;c-myc的表达自用药6h时起显著升高,12、24h最明显。结论罗非昔布可抑制人胃癌细胞株SGC7901的生长,其可能的作用机制之一为诱导细胞高表达c-myc基因。
Objective To explore the potential role of rofecoxib,a selective COX-2 inhibitor,on the proliferation of gastric adenocarcinoma cell line SGC7901.?Methods MTT colorimetric assay and proliferating cell nuclear antigen (PCNA) immunocellularchemical staining were used to study the proliferation of SGC7901 cells.The effects of rofecoxib on the expression of gene c-myc was studied by immunocellularchemical staining.?Results 1×10^-5 ～1×10^-9 mol/L rofecoxib exerted inhibitory action on SGC7901 cells,with the maximum inhibitory rate 35.46% produced by 1×10^-5 mol/L rofecoxib.Rofecoxib also decreased the expression of PCNA and increased the expression of oncogene c-myc,with the increase detectable 6 h after rofecoxib and becoming significant at 12 h and 24 h.?Conclusion Rofecoxib may inhibit the proliferation of gastric adenocarcinoma cells SGC7901 by enhancng the expression of oncogene c-myc.
出处
《徐州医学院学报》
CAS
2005年第3期203-206,共4页
Acta Academiae Medicinae Xuzhou
