摘要
本文系统地分析了中国药典９０版庆大霉素Ｃ组份的ＨＰＬＣ分析方法中流动相组成对庆大霉素Ｃ组份保留行为的影响，在ＳｐｈｅｒｉｓｏｒｂＣ＿（１８）ＯＤＳ柱上，最佳时流动相组成为０．０５ｍｏｌ／Ｌ的庚烷磺酸钠：冰醋酸：甲醇：Ｈ＿２Ｏ＝４．７：１．３：７３：２１，此时各组份的相对保留时间约分别为０．１３（衍生化试剂），０．２７（Ｃ＿１组份），０．６５（Ｃ＿１ａ组份），０．８５（Ｃ２ａ组份）和１．００（Ｃ＿２组份）。根据实验结果，对流动相中各组份在色谱分离过程中的作用进行了讨论。
The influence of mobile phase on the retention behavour of components C of Gentamicin was studied on the HPLC system employ-ed by Chinese Pharmacopia(1990).The optimum mobile phase was 0.05 mol/L sodium heptanesulphonate:glacial acetic acid:methanol:water=4.7:1.3:73:21 and the retention time of component pcaks with reiativ retent-ion times of about 0.13 (reagent),0.27(component C1),0.65(component C1a),0.85(component C2a)and 1.00(component C2)was about within 30 minutes on Spherisorb C18 ODS colunm(4 × 200mm).Also the mechanism of each component to the mobile phase in the chromatography was discussed based on experimental results.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
1994年第1期21-24,共4页
Chinese Journal of Antibiotics
关键词
高效液相色谱
庆大霉素
High performance liquid chromatography
Ion-pair re-versed-phase high performance liquid chromatography
Gentamicini Chin。ese pharmacopia(1990)
